Revealing the anaerobic acclimation of microbial community in a membrane bioreactor for coking wastewater treatment by Illumina Miseq sequencing
Jiadi Zhu, Lujun Chen, Yan Zhang*, Xiaobiao Zhu. 全文下载
School of Environment, Tsinghua University, Beijing 100084, China. E-mail: a5130751@126.comZhejiang Provincial Key Laboratory of Water Science and Technology, Department of Environmental Technology and Ecology,Yangtze Delta Region Institute of Tsinghua University, Zhejiang, Jiaxing 314050, ChinaSchool of Environmental and Civil Engineering, Jiangnan University, Wuxi 214122, China
College of Chemical Engineering, Beijing University of Chemical Technology, Beijing 100029, China
The dynamic change of microbial community during sludge acclimation from aerobic to anaerobic in a MBR for coking wastewater treatment was revealed by Illumina Miseq sequencing in this study. The diversity of both Bacteria and Archaea showed an increase–decrease trajectory during acclimation, and exhibited the highest at the domestication interim. Ignavibacteria changed froma tiny minority (less than 1%) to the dominant bacterial group (54.0%) along with acclimation. The relative abundance of Betaproteobacteria kept relatively steady, as in this class some species increased coupled with some other species decreased during acclimation. The dominant Archaea shifted from Halobacteria in initial aerobic sludge toMethanobacteria in the acclimated anaerobic sludge. The dominant bacterial and archaeal groups in different acclimation stages were indigenous microorganisms in the initial sludge, though some of them were very rare. This study supported that the species in “rare biosphere” might eventually become dominant in response to environmental change.© 2017 The Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences.
Microbial community; Acclimation; Coking wastewater; Anaerobic MBR; Miseq sequencing
Journal of Environmental Sciences
Elsevierjournal
10.1016/j.jes.2017.06.003
2018
139-148
Elsevier B.V.
