Deep exploitation of refractory organics in anaerobic dynamic membrane bioreactor for volatile fatty acids production from sludge fermentation: performance and effect of protease catalysis.
Hongbo Liu, Ling Wang, Bo Yin, Bo Fu, He Liu*. 全文下载
Jiangsu Key Laboratory of Anaerobic Biotechnology, School of Environmental and Civil Engineering, Jiangnan University, 214122 Wuxi, PR China
Jiangsu Collaborative Innovation Center of Technology and Material of Water Treatment, Suzhou 215011, PR China
Volatile fatty acids (VFAs) production from waste activated sludge fermentation could be improved in anaerobic dynamic membrane bioreactor (ADMBR) by retaining residual organics within the reactor and prolonging their reaction time. However, the accumulation of refractory organics made it operate unstably. Therefore, protease catalysis was adopted to deeply exploit those refractory organics in sludge. By combining with dynamic membrane retention, protease catalysis indeed presented a good performance. VFAs yield was further enhanced by over 40% in ADMBR. Membrane fouling was slightly relieved due to protein and polysaccharide degradations in the sludge of dynamic membrane. It was also interestingly found that not only protease activity of sludge was improved from 5 to 21 U/ml, but also β-GLC activity was enhanced from 13 to 20 μmoL/L/h. Microbial community analysis showed protease addition could reduce bacterial richness and evenness in sludge, and accelerate the growth of polysaccharides-hydrolyzing bacteria, as well as inhibit some proteolytic bacteria.
Protease catalysis; Waste activated sludge; Anaerobic fermentation; Dynamic membrane bioreactor; Refractory protein
Journal of Environmental Management
Elsevierjournal
10.1016/j.jenvman.2018.03.103
2018
478-785
Elsevier Ltd
