Laboratory of Environmental Biotechnology, School of Environmental and Civil Engineering, Jiangsu Key Laboratory of Anaerobic Biotechnology, Jiangnan University, Wuxi 214122, PR China; 2Jiangsu Collaborative Innovation Center of Technology and Material of Water Treatment,Suzhou 215011, PR China. |
ABSTRACT:A strictly anaerobic, Gram-staining-positive, spore-forming rod-shaped bacterium, and designated BXXT, was isolated from cow manure. Colonies on DSMZ medium 311c agar plates were cream, circular, opaque and lustrous. Growth occurred at 20–45 ℃ with a pH range of 5.0–10.0 and at NaCl concentrations of up to 2% (w/v). The optimum temperature, pH and NaCl concentration for growth were 30 ℃ pH 7 and 1% (w/v), respectively. The major cellular fatty acids were C16 : 0(26.8 %),C14 : 0(22.8 %), summe feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) (16.4 %) and C16 : 1ω9c (10.7 %). The main polar lipids of BXXT were diphosphatidylglycerol, phosphatidylethanolamine, unidentified aminolipids, an unidentified phospholipid and unidentified lipids. Acetate was mainly produced from H2/CO2, H2/CO2/CO (4/3/3, v/v/v), formate, glycerol, 1,2-propanediol,pyruvate, D-fructose and 2-methoxyethanol. BXXTis most closely related to Clostridium thermobutyricum DSM 4928T,Clostridium homopropionicum DSM 5847Tand Clostridium thermopalmarium DSM 5974Twith 16S rRNA gene sequence similarities of 96.9, 96.6 and 96.5 %, respectively. The DNA G+C content of BXXTwas 33.7 mol%, which was lower than that of C. thermobutyricum DSM 4928T(37.0 mol%) and C. thermopalmarium DSM 5974T(35.7 mol%). In addition, DSM 4928Tand DSM 5974Tare thermophilic members of the genus Clostridium. The absence of C15 : 0 also distinguished BXXTfrom Clostridium thermobutyricum. On the basis of phylogenetic, phenotypic and chemotaxonomic evidence, the novel isolate represents a novel species within the genus Clostridium, for which the name Clostridium bovifaecis sp. nov is proposed. The type strain of the type species is BXXT(=JCM 32382T=CGMCC 1.5228T). |
